The Netherlands BAG includes structural biology and enzymology groups situated at six Dutch universities (Amsterdam, Eindhoven, Groningen, Leiden, Utrecht and Wageningen) and at the Netherlands Cancer Institute in Amsterdam, with a total of fifteen PIs. While the structural biology research projects carried out in these groups are highly diverse, they can be largely classified into two major themes: (i) research on proteins and systems with a strong biomedical relevance and (ii) research concerning the design of new biocatalysts for green chemistry and new-to-nature reactions. Projects deal with prokaryotic and eukaryotic proteins, membrane and soluble proteins, cell surface receptors, and protein-protein, protein-ribonucleotide, protein-drug and enzyme-ligand complexes.