The VosA-VelB hetero-dimeric complex plays a pivotal role in regulating development and secondary metabolism in Aspergillus nidulans. VadH is a new VosA/VelB-activated gene which is involved in regulating asexual/sexual development, osmotic stress response, and ST production in A. nidulans. The transcriptome between the vadH-deletion mutant and Aspergillus nidulans FGSC4 (WT) were analyzed by RNA-suquencing in three phrases: vegetative growth, asexual development and sexual development.