This dataset is a component of the PAN-Arctic data collection of benthic BIOtas (PANABIO) and has been collated during the Census of Marine Life project Arctic Ocean Diversity (ArcOD; http://www.arcodiv.org/). It contains a total of 650 abundance and wet biomass records of 207 macro- and mega-benthic taxa (species to family level) identified in 18 Agassiz trawl catches and 25 van Veen grab samples taken at 43 stations located in the Beaufort Sea at water depths of 60–1,072 m during one cruise in 2009. The dataset is also available in a PostgreSQL-based data warehouse that can be accessed and queried through an open-access frontend web service at https://critterbase.awi.de/panabio.