Replication Data for: Evaluation of the cytotoxic and antimicrobial potential of epiphytic biomass obtained from Laminaria hyperborea biorefinery side-streams

Dataset

DOI

This dataset describes the calculations and the related methods used to determine the EC50 values-cytotoxicity against 5 cell lines (MOLM13, PC3, MCF7, NRK, H9C2) of the crude extracts and flash fractions of Laminaria's epiphytes. In order to assess the cytotoxicity, we performed the WST-1 cell proliferation assay, a colorimetric assay, using 96 well plates [1]. By measuring the UV absorbance, we calculated the % cell survival and then, the EC50 (concentration that reduces the viability of cells by 50%) using four-parameter regression analysis (dose response curve). We also performed one-way statistical analysis to determine differences between the extracts and between the fractions.

[1] Diaz, A.E.C.; Herfindal, L.; Andersen, H.L.; Fossen, T. Cytotoxic Natural Products Isolated from Cryptogramma Crispa (L.) R. Br. Molecules 2023, 28, 7723, https://doi.org/10.3390/molecules28237723

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Identifier
DOI	https://doi.org/10.18710/WQIZAX
Metadata Access	https://dataverse.no/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.18710/WQIZAX

Provenance
Creator	Barouti, Angeliki
Publisher	DataverseNO
Contributor	Barouti, Angeliki; University of Bergen
Publication Year	2024
Funding Reference	Research Council of Norway RCN project numbers # 234506, #261669, and #30959 ; This research was funded by the Research Council of Norway (RCN project numbEuropean Council JPI-AMR RCN project #298410
Rights	CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess	true
Contact	Barouti, Angeliki (University of Bergen)

Representation
Resource Type	tabular; Dataset
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Version	1.0
Discipline	Chemistry; Life Sciences; Medicine; Natural Sciences