PAM fluorometry monitoring symbiosis stability of Symbiodiniaceae in the foraminifera Sorites orbiculus

DOI

The photosystem II efficiency of the symbiotic algae of Sorites orbiculus was measured with PAM fluorometry in an experiment, assessing the long-term stability of newly established symbiotic associations. Therefore, specimens were cultivated for 14 days following menthol-DCMU bleaching and re-inoculation with Symbiodinium microadriaticum (strains KB8 and CCMP2467) and Fugacium kawagutii (strain F2) individually and collectively. The final re-inoculation measurement also served as the initial stability measurement. Accordingly, this measurement is included in both data tables and is provided here under the experimental treatment label "re-inoculation." Specimens were cultivated in 6-well plates, with 12 samples containing 4 replicates and 2 samples containing 2 replicates per treatment / control. The medium, supplemented with a Nannochloropsis food mixture, was exchanged twice weekly. Control groups included non-bleached specimens and bleached specimens maintained with or without 5 µmol L-1 DCMU in artificial seawater (ASW). Environmental conditions were maintained at 22 - 24°C temperature, 37.5 - 40.5‰ salinity, and 46 - 63 µmol photons cm-2 s-1 illumination under a 12:12 h light-dark cycle.

Symbiodinium microadriaticum, strain CCMP2467: collected in the Gulf of Aquaba on 2004-07-30, symbiont isolated from Stylophora pistillataSymbiodinium microadriaticum, strain KB8: collected in Hawaii, symbiont isolated from Cassiopaia xamanchaFugacium kawagutii, strain F2: collected in Jamaica, Caribbean, symbiont isolated from Meandrina meandrites

Identifier
DOI https://doi.org/10.1594/PANGAEA.984043
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.984033
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.984043
Provenance
Creator Timme, Lukas Theodor ORCID logo; Henschen, Helena; Puerto Rueda, Diana N ORCID logo; Manda, Sneha; Parkinson, John Everett ORCID logo; Wild, Christian ORCID logo; Abramovich, Sigal ORCID logo; Morard, Raphael; Schmidt, Christiane ORCID logo
Publisher PANGAEA
Publication Year 2026
Funding Reference Deutsche Forschungsgemeinschaft, Bonn https://doi.org/10.13039/501100001659 Crossref Funder ID 444059848 https://gepris.dfg.de/gepris/projekt/444059848 SYMBIOAID: the role of diatom endosymbionts on the adaptive potenital of benthic foraminifera to climate change
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 8568 data points
Discipline Earth System Research
Spatial Coverage (34.917 LON, 29.502 LAT); Gulf of Aqaba
Temporal Coverage Begin 2023-10-24T00:00:00Z
Temporal Coverage End 2023-11-07T00:00:00Z