Archaeorhizomycetes is a class of globally widespread soil-dwelling fungi, originally proposed to be associated with plant roots, but their ecology and nutritional mode are not clearly defined. Here, we have sequenced and aligned approximately 2500 bp of the ribosomal DNA from 18S (covering the V4 region), ITS and well into 28S. Sequences have been aligned using the 'linsi' algorithm in MAFFT v7.505 (Katoh & Standley, 2013). The alignment was trimmed for ambiguously aligned positions with trimAl using the –gappyout option (Capella_Gutiérrez et al., 2009).The phylogenetic tree was constructed with IQ-TREE v2.2.2.3 (Nguyen et al., 2015). The best fitting model was determined to be GTR+F+I+R5 by ModelFinder (Kalyaanamoorthy et al., 2017), and support values for the tree were calculated with the SH-like approximate likelihood ratio test (aLRT) and ultrafast bootstrap approximation UFBoot (Hoang et al., 2018), both with 1000 replicates.

MAFFT, v. 7.505

IQ-TREE, v. 2.2.2.3