Interaction of sugars and a model peptide

DOI

We propose to perform a NDIS experiments at the SANDALS diffractometer at T = 300 K on 7 aqueous solutions of NMA and trehalose and NMA and glucose, aimed at investigating the interaction of the two sugars with N-methylacetamide in solution. We have previous evidence of strong differences in the hydration properties of the two sugars, which may have a counterpart in the interaction of sugars with NMA, where NMA is a model system for the amide linkage of peptides and proteins. Investigating the interaction of this small molecule with trehalose and glucose in aqueous solution may address the longstanding question about the highest effectiveness of trehalose as a bio-protectant, compared to glucose. Indeed it is not yet clear if trehalose exploits its action by substituting water in the protein hydration shell, or not.

Identifier
DOI https://doi.org/10.5286/ISIS.E.61781319
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/61781319
Provenance
Creator Dr Laura Maugeri; Professor Maria Antonietta Ricci; Professor Fabio Bruni; Dr Sylvia McLain; Dr Silvia Imberti; Dr Roberta Angelini; Dr Barbara Ruzicka; Dr Valentina Nigro
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Natural Sciences; Physics
Temporal Coverage Begin 2015-06-19T08:00:00Z
Temporal Coverage End 2015-06-22T11:26:01Z