In our article “Production of 11-oxygenated androgens by testicular adrenal rest tumors” (https://doi.org/10.1210/clinem/dgab598) we defined the production of 11-oxygenated 19-carbon steroids by testicular adrenal rest tumors (TART) in men with 21-hydroxylase deficiency (21OHD), by the quantification of these steroid hormones in the spermatic vein blood and corresponding peripheral vein blood samples of men with 21OHD. Eleven steroid hormones were quantified in left (n=7) and right (n=4) spermatic vein- and simultaneously taken peripheral blood (n=7) samples from seven men with 21OHD and TART using liquid chromatography-tandem mass spectrometry. These samples were previously obtained by Claahsen-van der Grinten et al. (DOI:10.1210/jc.2007-0337). All patients had bilateral TART, but for three patients (2, 3, and 7) no material was longer available from the right spermatic vein. For comparison, we also measured the peripheral steroid concentrations in five adrenalectomized patients and twelve age, sex, and BMI-matched controls. Additionally, steroids were measured in previously collected media (Turcu et al. DOI:10.1210/jc.2015-1023) of in vitro cultured TART cells and adrenal cells, that were treated for 24 or 48 hours with adrenocorticotropic hormone (ACTH; 10 nmol/L) or Luteinizing Hormone (LH; 25 ng/mL).In this repository we provide the raw data files and the supplementary data files. The abbreviations of the quantified steroid hormones are listed in “Overview.pdf”.The raw data can be found in three different files:Schroder_2021_peripheral_vein.csv contains the peripheral steroid hormone concentrations (ng/dL) of seven men with 21OHD and TART (P1-P7), five adrenalectomized controls (C1-C5), and twelve BMI- and age-matched healthy men (M1-M12).Schroder_2021_spermatic_vein.csv contains the steroid hormone concentrations (ng/dL) in left (n=7) and right (n=4) spermatic vein blood samples of seven men with 21OHD and TART. The corresponding numbers of the spermatic vein samples and peripheral vein samples belong to the same patient.Schroder_2021_TART_Adrenal.csv contains the steroid hormone concentrations (pg/mL) in TART-cell- or normal adrenal cell-conditioned medium under basal conditions or 24 or 48 hours of treatment with ACTH or LH.Three supplementary Tables are provided:Schroder_2021_SupplementaryTable1.pdf contains Supplementary Table 1, presenting the concentrations (nmol/L) of eleven steroid hormones in vena spermatica (v.Sp) and peripheral plasma (P) samples with corresponding ratios (R) of seven men with 21OHD and TART with left and/or right cannulated spermatic veins. Steroid concentrations below the lower limit of quantification (LLOQ) were set at 0.5*LLOQ for calculation of the ratios.Schroder_2021_SupplementaryTable2.pdf contains Supplementary Table 2, presenting the concentrations (nmol/L) of steroid hormones in TART-cell- or normal adrenal cell-conditioned medium under basal conditions or after 24 hours of treatment with ACTH.Schroder_2021_SupplementaryTable3.pdf contains Supplementary Table 3, presenting the concentrations (nmol/L) of steroid hormones in TART-cell- or normal adrenal cell-conditioned medium under basal conditions or after 48 hours of treatment with ACTH.