16S rDNA amplicon sequencing of Dermacentor nuttalli ticks

Amplification of V4-V5 hypervariable regions was performed as conventional PCR procedure. The primers 515F (5'- GTG CCA GCM GCC GCG GTA A -3') and 907R (5'- CCG TCA ATT CCT TTG AGT TT -3') used in amplifications also contained an 8 bp multiplex barcode and Illumina adapters.

Identifier
Source https://data.blue-cloud.org/search-details?step=~01237B12847744CE40CADA9610B7EE9BE75B0DA588A
Metadata Access https://data.blue-cloud.org/api/collections/37B12847744CE40CADA9610B7EE9BE75B0DA588A
Provenance
Instrument Illumina HiSeq 2500; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2017-01-01T00:00:00Z