In conclusion, we sequenced E. superba transcriptome at different temperatures using high-throughput Illumina sequencing technology, which provided massive genetic resources, including 596378 unigenes and 1006543 transcripts. In addition, analysis of differential gene expression revealed more genes expressed in LT vs MT than in LT vs HT and MT vs HT. Differentially expressed temperature-related genes were mainly involved in ABC transporters, MAPK signaling pathway, Hippo signaling pathway and cAMP signaling pathway. This work provides a good foundation for further research of temperature adaptation mechanism in E. superba.