Assembly Free Viral Genomes from Station ALOHA

We developed an assembly-free, single-molecule nanopore sequencing approach enabling direct recovery of high-quality viral genome sequences from environmental samples. Applied to samples from Station ALOHA in the North Pacific Subtropical Gyre, the method yielded full-length double-stranded DNA virus genome sequences and revealed viruses not fully recovered using short read assembly approaches in the same samples. Additionally, novel virus-like DNA sequences were discovered, whose structures, gene contents and concatemer lengths suggested they represent phage-inducible chromosomal islands packaged in discrete concatemeric lengths within phage particles.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0126D86E08CF2C1111E2FA1223278A16E035E31BD9E
Metadata Access https://data.blue-cloud.org/api/collections/6D86E08CF2C1111E2FA1223278A16E035E31BD9E
Provenance
Instrument NextSeq 500; GridION; ILLUMINA; OXFORD_NANOPORE
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (-160.844W, 22.307S, -156.800E, 24.559N)
Temporal Coverage Begin 2018-04-02T00:00:00Z
Temporal Coverage End 2019-09-06T00:00:00Z