This dataset is a component of the PAN-Arctic data collection of benthic BIOtas (PANABIO) and has been collated during the Census of Marine Life project Arctic Ocean Diversity (ArcOD; http://www.arcodiv.org/). It contains a total of 1,321 abundance and wet biomass records of 53 macrobenthic taxa (species to genus level) identified in 101 van Veen grab samples taken at 29 stations located in the Beaufort Sea at water depths of 32–49 m during two cruises in 1986 and 2002, respectively. The dataset is also available in a PostgreSQL-based data warehouse that can be accessed and queried through an open-access frontend web service at https://critterbase.awi.de/panabio.