The present hyperspectral imaging (HSI) database is composed of healthy leaves and leaves infected with Zymoseptoria tritici fungal pathogen responsible for Septoria tritici blotch (STB). Leaves of two durum wheat genotypes were studied under controlled conditions to track the evolution of STB disease and capture significant spectral and spatial differences until the onset of symptoms. Hyperspectral image acquisitions were purchased with two cameras in visible-near infrared (VNIR) and short-wave infrared (SWIR) spectral ranges on eighteen dates between one day before inoculation and twenty days after inoculation. For each wavelength range studied, a total of 1175 images provided information on 3326 leaves measured throughout the experiment.