Gephyrocapsa huxleyi growth rates during Thermal performance curves after exposure to marine heatwave laboratory experiment

DOI

Cultures of Gephyrocapsa huxleyi were isolated from the Subantarctic water off the east coast of New Zealand in December 2023, and exposed to a simulated marine heatwave. Thermal performance curves (TPC) were produced at two time points after the heatwave. The first time (T1) was immediately after the heatwave incubations, and the second (T2) was five weeks after the heatwave incubations. During the five weeks in between experiments, both treatments were held under constant control conditions (12.2°C). Triplicates of the Control and Heatwave populations were grown in 30mL polycarbonate tubes (Oakridge) in Aquil medium (Price et al., 1989). The tubes were incubated in aluminium heatblocks (Thomas et al., 1963), with circulating water baths (Julabo), cooling one end and heating the other to create a thermal gradient with 10 temperatures from 10-20°C. The block was lit from below at ~85 µmol(photons)/m²/s on a 12:12 light:dark cycle. To account for minor variation in light levels, the replicates were rotated through the block each day at the same temperature. Chlorophyll was measured daily using a Turner 10-AU fluorometer. Growth rates were calculated from the slope of the natural log of the chlorophyll a against time while the replicates were in the exponential growth phase. The data was collected and analysed in 2024 as part of a Master's research project investigating the impacts of marine heatwaves on subantarctic phytoplankton.

Identifier
DOI https://doi.pangaea.de/10.1594/PANGAEA.995564
Related Identifier References https://doi.org/10.1080/01965581.1988.10749544
Related Identifier References https://doi.org/10.4319/lo.1963.8.3.0357
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.995564
Provenance
Creator Powell, Jessica
Publisher PANGAEA
Publication Year 2026
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints); https://creativecommons.org/licenses/by/4.0/
OpenAccess false
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 1320 data points
Discipline Design; Fine Arts, Music, Theatre and Media Studies; Humanities
Spatial Coverage (170.512W, -45.865S, 171.541E, -45.836N); New Zealand
Temporal Coverage Begin 2023-12-18T02:00:00Z
Temporal Coverage End 2024-09-28T01:00:00Z