The Lewis Run relocateable aquatic site is a small 2nd order wadeable stream located about 60 miles west of Washington DC in Clarke County, Virginia. The majority of the stream reach flows past and through agricultural lands on the Casey Trees property. This site is one of two aquatic sites found within Domain 02, the Mid-Atlantic. This site has one meteorological station located in the riparian area. The met station is outfitted with a subset of the same sensors used at terrestrial sites. Measurements include wind speed and direction, air temperature, barometric pressure, relative humidity, shortwave radiation, and PAR. This site has one upstream sensor station and one downstream sensor station. Measurements include PAR, temperature, water quality [specific conductivity, chlorophyll a, dissolved oxygen content, pH, turbidity, and fluorescent dissolved organic matter (only downstream)], and nitrate is measured at the downstream station. Eight groundwater wells throughout the site collect specific conductivity, water tempertaure, and elevation of groundwater. Field ecologists collect the following types of observational data at this site: aquatic organisms (Aquatic Microbes (surface water), macroinvertebratesmicroalgae, plants and macroalgae, zooplankton. Biogeochemical data on plants,sediment water and particulates. As well as physical aquatic data on bathymetric maps, stage,secchi depth and depth profiles, and riparian assessment.Total data products planned for this site: 78