Lewis Run (LEWI) is an aquatic NEON field site located about 60 miles west of Washington, D.C. in Clarke County, Virginia. Lewis Run is a small wadeable stream that drains a 11.9 km2 (2940 acre) watershed. The majority of the stream reach flows past and through land managed by Casey Trees, a nonprofit organization that raises trees for planting in and around the Washington, D.C. area. The surrounding region is characterized by general land use types including successional fields, pastures, woodlands, and small ponds. This site is located within NEON's Mid-Atlantic Domain (D02), a densely populated region bounded by the Atlantic Ocean on the east and stretching down the Eastern Seaboard from southern New Jersey to northern Georgia. The Mid-Atlantic Domain includes one other aquatic site and two terrestrial sites. LEWI is located near the BLAN terrestrial site.