This dataset was exctracted from time-lapse microscopy data for spatio-temporal analysis of Cancer Stem Cells in a cell population. Live cells were imaged for 5 days in a atmosphere and temperature controlled top stage incubator. The cell lines were SUM159PT or MDAMB231. The pALDH1a1-mNeptune reporter was used as CSC marker. Highly fluorescent cells are considered as CSC while low fluorescence signal indicate differentiated cell. see Bidan, N., Bailleul‐Dubois, J., Duval, J., Winter, M., Denoulet, M., Hannebicque, K., ... & Lagadec, C. (2019). Transcriptomic analysis of breast cancer stem cells and development of a pALDH1A1: mNeptune reporter system for live tracking. Proteomics, 19(21-22), 1800454. Cells were stained with Hoechst to allow segmenatation and tracking.

The proposed dataset comprises : 1) RAW DATA : positions and fluorescent state of all single cell in 7mmx7mm field of view in both .csv and .mat files 2) intermediate analysis in matlab (including analysis scripts) 3) FIGURES : for spatio-temporal analysis of Cancer Stem Cell plasticity in both .csv and .mat files