Nine experimental vineyards were sampled in the Rhein-Hesse area, Germany, in June 2017. Each vineyard exhibited four experimental plots with different soil management types (complete cover, herbal seed mixture cover, alternating tillage, and complete tillage).In two inter-rows of each vineyard, a pooled sample of eight soil cores of the upper soil layer (0-10 cm) was used for DNA extraction. The samples were analyzed using Illumina Sequencing by an external sequencing company. The resulting data were further processed to obtain a matrix of OTU and read counts for soil bacteria and soil fungi. Shannon Indices were calculated from these matrixes as metrics of bacterial and fungal diversity.