RS5 is a nickel-based superalloy used in the manufacture of large cast jet engine components. Scatter observed in fatigue test results makes it difficult for the aerospace industry to determine the fatigue life of such components. Once validated, crystal plasticity finite element modelling (CPFEM) is a powerful technique that can be used to predict this scatter. We aim to use Bragg edge neutron transmission to image the changes in elastic strain distribution of different test samples under two different loading conditions. These experiments will provide grain scale elastic strain measurements that will be used for the validation and development of a CPFE model. This will be an important first step towards models that can account for the scatter in fatigue tests of these important materials, opening doors for the application of CPFEM to the prediction of fatigue life.