The dataset contains records of the Iberian Wolf (Canis lupus) collected in the lower valley of River Sabor (NE Portugal), in the scope of the long-term Ecological Monitoring Program (PME) of the Baixo Sabor Hydroelectric Dams (AHBS), promoted by EDP – Energias de Portugal, S.A. This dataset includes results of surveys made in and around the area affected by the dam, from February 2010 to September 2014. Sampling was performed in transects distributed in the area of the lower Sabor river valley (and some tributaries) and in rivers Maçãs and Angueira, from the river mouth up to 42 km upstream. The presence and identity of the target species were assessed through presence signs, mainly scats. However, since the scats attributed to the wolf were not validated with molecular tools to screen for possible confusion with those of domestic dogs, their taxonomic identification in this dataset is presented at the genus level.