French Guiana is recognized as a biodiversity hotspot, particularly with respect to its ichthyofauna. This overseas department hosts 416 fish species, including 366 strictly freshwater taxa (Lebail et al., 2012), representing a richness 5 to 6 times higher than that of metropolitan France. The RezoFleuve program, coordinated by the DECOD Joint Research Unit (UMR DECOD, INRAE), was initiated in response to requests from local institutions: the French Guiana Water Office (OEG), the Amazonian Park of French Guiana (PAG), the DGTM (French Guiana Prefecture), and the Hydreco laboratory. The objective of RezoFleuve is to generate scientific knowledge to establish reference trophic networks, based on three major study sites: Saut Mapaou on the Approuague River, Abattis Cottica, and Saut Lavaud on the Maroni River, which forms the western border with Suriname. This innovative program employs a combination of methodologies, including stable isotope analysis (carbon and nitrogen) and dietary metabarcoding of captured individuals. The outcomes are expected to inform management strategies for fishery resources. As a prerequisite to RezoFleuve, UMR DECOD, in collaboration with Hydreco, OEG, and DGTM, developed an Information System that compiles all historical WFD (Water Framework Directive) data for French Guiana (https://eseweb.rennes.inrae.fr/RezoFleuve/). The deposited dataset comprises all fish captures conducted at the three RezoFleuve study sites and serves as a reference for the identification of the biological samples from collected specimens (not included here).