This study investigated the knowledge, attitude, and perceptions of pregnant women, their family members, healthcare workers and decision-makers on integrated antenatal screening. On the demand side, we conducted 26 semi-structured in-depth interviews with pregnant women, their husbands, and mothers-in-law in Kapilvastu and Kathmandu. On the supply side, we conducted 11 interviews with health workers involved in antenatal screening and local and national health system decision-makers.