Polymer-lipid nanodisc adsorption at the solid-liquid interface

DOI

Nanostructured lipid aggregates offer enormous potential as tools for membrane structural biology and biophysics. Poly(Styrene-Maleic Acid) stabilized lipid nanodiscs can be easily prepared, without detergent, at low cost. In this proposal we wish to study the adsorption of polymer-stabilised lipid nanodiscs at a range of solid-liquid interfaces in order to determine whether they can be close-packed as intact discs at these surfaces, to provide oriented membrane protein supports which can assist structure determination. We will study the adsorption of the discs to bare silica (negative), aminosilane functionalised silica (positive) and a silica surface containing a pre-deposited phospholipid bilayer to assess whether formation of a floating nanodisc layer can be prepared. We will also vary the nanodisc charge by altering the lipid composition & salt concentration in the buffer solution.

Identifier
DOI https://doi.org/10.5286/ISIS.E.42593130
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/42593130
Provenance
Creator Mr Nicolas Bertram; Professor Karen Edler; Dr Thomas Arnold; Dr Rob Barker; Dr Gavin Hazell
Publisher ISIS Neutron and Muon Source
Publication Year 2016
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2013-10-16T23:00:00Z
Temporal Coverage End 2013-10-21T23:00:00Z