At the EPN campus level structural biology work is carried out in all the 4 institutes (IBS, ESRF, ILL and EMBL). Today all structural biology groups are turning to cryo-EM. The EM demand is therefore extremely high and present in the 4 Institutes. However there are also some other Research Institutes in Grenoble and we are now proposing that the BAG is extended to them (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB) In a nutshell, there are around 40 PIs that could benefit from Krios time on the EPN campus (more than 100 different samples). 2 Glacios are available on the EPN campus for pre-screening. Whereas some real structural work could be carried out on Glacios microscopes, for difficult samples the use of a krios is indispensable (heterogeneous sample or limited concentration requiring a large number of images; small protein etc). Only these samples will be selected for Krios time at the BAG level.