Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions

DOI

Degradation in Ulva lacinulata and reproduction in Ulva compressa were observed and described for four weeks. The natural occurrence of protoplasts in both species was found. Protoplast yields were calculated each week for several weeks. At the beginning of the experiment, heterogeneity of the thallus was visible under the microscope. The diameter of the cells in the thalli that later became protoplasts were measured. A germination experiment was performed after protoplasts were collected. Germination rates were calculated based on the number of individuals visible by the naked eye at the end of the experiment. 2 % (U. lacinulata) and 4 % (U. compressa) of protoplasts germinated into two morphologies: unattached germlings and unattached discs. Because discs released gametes early in their development, their diameter was measured to estimate the gametes yields produced at the end of the experiment. During degradation of U. lacinulata, we found an increase in biomass after the first two weeks of the experiment. Fresh weight measurements were taken each week and relative growth rates were calculated. Our results provide the first evidence of the natural production of protoplasts in Ulva spp. and estimate that higher seeding yields can be obtained by natural protoplast production than direct gametogenesis.

Identifier
DOI https://doi.pangaea.de/10.1594/PANGAEA.972125
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.972125
Provenance
Creator Cardoso, Isabel ORCID logo; Bartsch, Inka ORCID logo; Valentin, Klaus ORCID logo; Steinhagen, Sophie ORCID logo; Buck, Bela Hieronymus ORCID logo; Hofmann, Laurie C ORCID logo
Publisher PANGAEA
Publication Year 2025
Funding Reference Federal Ministry of Food and Agriculture https://doi.org/10.13039/501100005908 Crossref Funder ID 281DL02B20 https://foerderportal.bund.de/foekat/jsp/SucheAction.do?actionMode=view&fkz=281DL02B20 Verbundprojekt: Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel (MakPakScaleUp) - Teilprojekt B; Swedish Research Council https://doi.org/10.13039/501100004359 Crossref Funder ID 2022-00331_Formas https://www.vr.se/english/swecris.html?project=2022-00331_Formas A manual for the use of sustainable marine resources – Definition of the cultivation potential and commercially relevant compounds of Sea Lettuces (Ulva spp.) along the Swedish coastline
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Bundled Publication of Datasets; Collection
Format application/zip
Size 5 datasets
Discipline Earth System Research
Spatial Coverage (-9.214W, 39.395S, 8.515E, 53.742N)
Temporal Coverage Begin 2021-01-04T12:00:00Z
Temporal Coverage End 2023-07-24T10:00:00Z