Dataset on the results of PDC, LDH and AHD enzymatic characterization, ADH, LDH and PDC reactions towards pH, lactic acid synthesis using 100% CO2 and synthetic gas mixture mimicking real off-gasses of blast furnace of iron&steel industry.

The data herein correspond to the enzymatic characterization of all the enzymes involved in the multi-enzymatic system as well as the study of the multi-enzymatic system using 100% CO2 or synthetic gas mixture mimicking real off-gasses of blast furnace of iron&steel industry.

Description of the methods used to collect and generate the data: -Single enzymatic reactions were carried out in bicarbonate buffer in 2mL eppendorfs. -Multi-enzymatic system reaction was carried out in a 50mL using 100% pure CO2, synthetic gas mixture mimicking real off-gasses of blast furnace of iron&steel industy under atmosferic pressure and 25ºC. -Activities were obtained applying activity tests based on spectrophotometrical methods, -GC for the analysis of ethanol, acetaldehyde and acetoin concentration -LC-MS for lactic acid quantification. -NADH were analysed by spectrophotometry

Information about instruments, calibration and standards: -Varian Cary 50 Bio UV-visible spectrophotometer (Agilent®). -Shimadzu GC 2010 system, using a Stabilwax-DA column (15 m x 0.33 x 1 µm) -Shimadzu LCMS-2010A using a ICSep 87H USP L17 (Transgenomic) column

Environmental or experimental conditions: Atmospheric pressure and 25ºC.