This dataset contains experimental data collected from controlled laboratory experiments to examine the effects of environmental stressors on biofilm microbial communities. The dataset includes measurements of both functional and structural biofilm variables. Specifically, the functional variables assessed include photosynthetic efficiency (Yeff), basal fluorescence (F0), enzyme activities (β-D-1,4-glucosidase, alkaline phosphatase, and leucine-aminopeptidase). The structural variables include chlorophyll-a concentration and algal community composition at the genus level.

The data also includes microbial gene expression, with qPCR quantification of key genes, including 16S rDNA (for bacteria and archaea) and 18S rDNA (for eukaryotes, mainly algae). Additionally, bacterial communities were characterized using high-throughput sequencing of the 16S rDNA gene. Pesticide concentrations in the water samples, as well as other water quality parameters such as nutrient concentrations, were also measured to assess the environmental conditions during the experiments.

The dataset was collected under various treatment conditions, including high temperature, pesticide exposure, and water level reduction, in both sequential and co-occurrence stressor combinations. The data are organized by experiment type (EXP1, EXP2), treatment conditions, and analysis type, providing a comprehensive resource for researchers studying microbial ecology, ecotoxicology, and environmental stress responses.