Bacterial communities are considered crucial for bioflocs technology (BFT)-based aquaculture system. To decipher how bacterioplankton community composition changes with shrimp growth, rearing water was collected from biofloc-based shrimp (Litopenaeus vannamei) aquaculture system, located in South Korea. The high-throughput pyrosequencing of 16S rRNA gene amplicons (targeting V1-V3 hypervariable regions) was then conducted to determine compositional variations that occur in rearing water microbiota along the various shrimp growth stages.