Codes, processed data and examples of analyses used in the publication: Reconstruction of macroglia and adult neurogenesis evolution through cross-species single-cell transcriptomic analyses.

This repository contains data generated in the Bally-Cuif lab through scRNA-seq of dissected zebrafish telencephala, as well as code used in their analysis. Included are datasets and illustrations obtained from reanalysis of other studies. Comparative analyses between these datasets were conducted to investigate the evolution of adult neurogenesis and of macroglia.

All the data present in this repository are derived from scRNA-seq analysis obtained from various species (zebrafish in our case, and species ranging from cnidarians to humans in the case of reanalyzed datasets). Our dataset was generated by dissecting zebrafish brains, enriching for adult neural stem cells and sequencing individual cells with the 10X Chromium v2 kit. Our aim was to gain insight into the physiology and heterogeneity of adult neural stem cells and compare zebrafish neural stem cells to those found in other species. Retrieved datasets were generated for various purposes but were used in this study to serve as basis for comparative evolution analyses.

R, 3.6.3

Cellranger, 3.0.1

Imaris, 10.1.0

Raw sequencing data for the dataset generated in our lab are freely accessible at : https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE225863

Sequencing data previously generated by other labs and reanalyzed during this study are available from the original publications listed in the "Jeu de données associés" subsection.