The silver pomfret (Pampus argenteus) is one of the most preferred commercial marine fish species in many Asian countries. Recently, the silver pomfret catch has greatly reduced because of overfishing, and culture and breeding of this fish are faced with many challenges. In 2018, we succeeded in breeding nearly a million young silver pomfret, but we still need to solve the feeding efficiency problem. In culturing process, we found that the fish showed significant appetite for jellyfish including Aurelia aurita and Rhopilema esculentum, which had been reported in previous study. To further investigate whether the amino acids in jellyfish have great effects on olfactory regulating appetite in silver pomfret, we utilized four different concentrations of three amino acids and betaine to stimulate silver pomfret and tested their attractant effect in diets.