Data is organized into three excel sheets of bovine Holstein embryo spent culture medium metabolome, from in vivo developed and in vitro produced in two different conditions: - IVD.xlsx: 90 in vivo developed embryos - IVP.xlsx: 761 in vitro embryos – SOF medium supplemented with 1% foetal calf serum; - IVP176.xlsx: 176 in vitro embryos – IVF Bioscience medium Embryos were recovered at Day-6 and cultured individually for 26 hours. Culture medium was analysed by H1-RMN spectrometry, embryos were staged, graded and sexed. Sheets format is as follows: Columns Description* A Identifiant B to Y Metabolites Z D6 stage AA D6 Grade AB D7 Stage AC D7 Grade AD Sex