The study aims to investigate the role and effectiveness of microorganisms in constructed wetlands that utilize reclaimed aggregates as a substrate. Constructed wetlands are engineered systems designed to mimic natural wetlands for wastewater treatment, and the choice of substrate material can significantly influence their performance. Reclaimed aggregates, often derived from recycled construction and demolition waste, offer a sustainable and cost-effective alternative to traditional substrates. The primary goal of the research is to understand how these reclaimed aggregates impact microbial communities within the wetland, particularly in terms of their diversity, activity, and ability to degrade pollutants. By analyzing the microbial composition and function, the study seeks to optimize the design and operation of constructed wetlands for enhanced pollutant removal, including nutrients, organic matter, and potentially harmful contaminants. The relevance of this research lies in its potential to promote sustainable wastewater treatment solutions that not only reduce environmental pollution but also recycle waste materials, contributing to circular economy principles. This approach could be particularly beneficial in urban and industrial settings where space and resources are limited, offering a green technology that aligns with environmental conservation and resource efficiency goals.