The goal was to characterize the rhizospheric bacterial communities induced by different plants to understand whether the colonization varies with plant species. Methods and Results: Rhizospheric soils and plant roots of Solanum melongena (E), Nicotiana benthamiana (T) and Echinochloa crusgali (G) including controls having no plant with no water (C0) and water (C) were collected from laboratory experiment for investigating bacterial communities through DGGE and 16S rRNA gene pyrosequencing. The results indicated that Bacillariophyta was the main genus in all soils except C0, whereas C0 was mainly dominated by Firmicutes, Actinobacteria and Proteobacteria. Sediminibacter and Gammaproteobacteria was also found in E and T rhizosphere respectively, whereas dominant clones only observed in G rhizosphere. In addition, two novel species (Streptomyces mirabilis sp. nov. and Pseudoxanthomonas mexicana) were detected in E and T plant roots.Conclusions: The results of this study confirm that rhizospheric bacterial community structure is plant species-specific, which led to plant species level study in future.