This megafauna presence/absence matrix was obtained by analysing seabed images collected during successive environmental surveys conducting with remotely operated vehicles. The surveys took place around six oil and gas installations in the Faroe-Shetland Channel, an area known to harbour deep-sea sponge grounds. The matrix is constituted by multiple Operational Taxonomic Units (OTUs) as well as environmental parameters such as temperature, bathymetry and slope. Eigenvectors (Listed as MEM1 to MEM15) resulting from a distance-based Moran's Eigenvector Mapping (dbMEM) applied on the data have also been included. Further details regarding the dataset can be found in the publication listed below.