This dataset includes 210Pb ages from a lake sediment core from central Alaska. The purpose of this study was to assess the sensitivity and validity of lacustrine faecal steroid reconstructions of human and animal presence within the landscape by combining analyses of modern faecal samples and a sediment archive from Chisholm Lake, Alaska, where historical and archaeological population changes are well documented. The lake sediment core was recovered from Chisholm Lake in the Tanana Valley of interior Alaska (64.302267 N, 146.687331 W) in September 2019 using a Bolivia corer. Lead-210 (210Pb) measurements were completed by Flett Research and ages were determined using a constant rate of supply (CRS). CRS-derived ages are reported for samples with activity levels that exceed sediment-supported 210Pb activity and used to develop a chronology for the Chisholm Lake (2019) core between 0 to 3 cm core depth (last c. 130 years).