Biological membrane mimics are a bottle neck in biophysical studies today. The need to develop advanced models that actually represent the properties of specific cellular membranes is imperative in order to advance the understanding of for example their role as barriers and for affinity studies with biomolecules in solution. Recently, there has been a boost in the development of complex membranes for models of bacterial membranes among others. In this work we would like to further contribute at the advancing of this field by creating mimics of mammalian membranes using deuterated lipid species extracted from various strains of E. coli: a genetically modified strain that produces PC and regular strains producing PE, PG and cardiolipin.