The study aimed to bridge the knowledge gap in evaluating ethyl acetate (EtOAc) as a single organic solvent in lipid extraction and contribute to a deeper understanding of the targeted organic solvent lipid extraction systems. The efficiency of the EtOAc method was benchmarked against the well-established Folch method on total lipid content, lipid class profile, and fatty acid composition of four marine species: Atlantic salmon (Salmo salar Linnaeus, 1758), the zooplankton Calanus finmarchicus (Gunnerus, 1770), the microalgae Porosira glacialis (Grunow) Jörgensen, 1905, and the macroalgae Saccharina latissima (Linnaeus). Gas Chromatography-Flame Ionization Detector (GC-FID) analyses indicated that these marine species are excellent sources of highly unsaturated fatty acids. Additionally, High-Performance Thin-Layer Chromatography (HPTLC) results demonstrated a synergistic relationship between solvent polarity and lipid polarity. Overall, the EtOAc method proves to be a potent alternative for extracting neutral lipids and free fatty acids from biological materials such as salmon fillets and C. finmarchicus. However, it exhibits lower efficiency with polar and complex lipids, as observed in P. glacialis and S. latissima.

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