Solution studies on Osteopontin calcium-phosphate nanoclusters

DOI

Osteopontin 1-149 is an intrinsically disordered polypeptide, which, under certain conditions, sequesters calcium phosphate (CP) forming a type of calcium phosphate nanocluster (CPN). The CPNs not only play essential regulatory biological roles but also have a number of potential applications, e.g. in the control of mineralisation of prostheses and implants and in the formulation of stable artificial biofluids. A previous SAXS study showed that the OPN-CPN comprised a core of calcium phosphate surrounded by a protein shell. We propose to study how different conditions during OPN-CPN affect CPN structure, by using a full contrast variation study on recombinant human OPN1-157.

Identifier
DOI https://doi.org/10.5286/ISIS.E.47624255
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/47624255
Provenance
Creator Professor Tommy Nylander; Dr Carl Holt; Miss Marianna Yanez; Dr Joe Zaccai; Dr Susana Teixeira; Dr Costanza Montis; Mr Samuel Lenton; Dr Michael Haertlein
Publisher ISIS Neutron and Muon Source
Publication Year 2017
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2014-06-17T23:00:00Z
Temporal Coverage End 2014-06-18T23:00:00Z