A detailed microbiological and geochemical study related to the alteration of basaltic glass of pillow lavas from the oceanic crust recovered from Hole 896A on the Costa Rica Rift (penetrating 290 m into the volcanic basement) has been carried out. A number of independent observations, pointing to the influence of microbes, may be summarized as follows: (1) Alteration textures are reminiscent of microbes in terms of form and shape. (2) Altered material contains appreciable amounts of C, N and K, and the N/C ratios are comparable to those of nitrogen-starved bacteria. (3) Samples stained with a dye (DAPI) that binds specifically to nucleic acids show the presence of DNA in the altered glass. Further, staining with fluorescent labeled oligonucleotide probes that hybridize specifically to 16S-ribosomal RNA of bacteria and archaea demonstrate their presence in the altered part of the glass. (4) Disseminated carbonate in the glassy margin of the majority of pillows shows delta13C values, significantly lower than that of fresh basalt, also suggests biological activity. The majority of the samples have delta18O values indicating temperatures of 20-100ºC, which is in the range of mesophilic and thermophilic micro-organisms.

Supplement to: Torsvik, Terje; Furnes, Harald; Muehlenbachs, Karlis; Thorseth, Ingunn H; Tumyr, Ole (1998): Evidence for microbial activity at the glass-alteration interface in oceanic basalts. Earth and Planetary Science Letters, 162(1-4), 165-176