Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013

The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates.The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of lipases and esterases in the extract was analysed fluorometrically after Knotz et al. (2006, doi:10.1016/j.cbpa.2006.07.019) using 4-methylumbelliferyl butyrate as substrate. Citrate synthase activity was analysed photometrically after Stitt (1984) modified by Saborowski and Buchholz (2002) with oxaloacetic acid as substrate.For detailed description please contact the author.

DOI https://doi.org/10.1594/PANGAEA.835177
PID https://hdl.handle.net/10013/epic.43161
Related Identifier https://doi.org/10.1594/PANGAEA.835181
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.835177
Creator Schmithüsen, Tosia; Niehoff, Barbara ; Auel, Holger
Publisher PANGAEA
Publication Year 2014
Funding Reference Seventh Framework Programme, 264933
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 3564 data points
Discipline Earth System Research
Spatial Coverage (-56.074W, 56.446S, -10.869E, 62.858N); North Atlantic
Temporal Coverage Begin 2013-03-25T13:24:00Z
Temporal Coverage End 2013-04-11T10:43:00Z