Microscopic interactions between water and urea and a beta-turn forming peptide

DOI

The mechanisms by which proteins fold and unfold in aqueous solutions is still not well understood. Depsite urea being a common protein denaturant, there is no atomic level understanding of how this molecule interacts with the functional groups on a protein or peptide in aqueous solution in order to cause unfolding. Here we proposed to measure the beta-turn forming pentapeptide -Tyr-Pro-Gly-Asp-Val-NH2 in water and in aqueous urea solutions in order to understand the details of how the folded peptide is hydrated and how this hydration is or isn't disrupted upon the addition of urea which will unfold the peptide in solution.

Identifier
DOI https://doi.org/10.5286/ISIS.E.49913525
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/49913525
Provenance
Creator Dr Sylvia McLain; Dr Ric Gillams; Dr Sebastian Busch; Ms Nicola Steinke; Dr Sam Callear; Dr Richard Gillams
Publisher ISIS Neutron and Muon Source
Publication Year 2017
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2014-08-05T23:00:00Z
Temporal Coverage End 2014-08-09T23:00:00Z