Hydrogen evolution activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge


Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogenase activity by means of an activity-based screen. The hydrogen evolution activity of protein extracts from positively screened clones was determined by means of gas chromatography. For the most active clone (SP11F2) the hydrogen evolution activity was determined at different pH conditions and the stability of the respective enzyme ectract under different storage conditions was tested. The datasets contain hydrogen evolution activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields.

Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity (Adam and Perner 2018). The hydrogen evolution activity of the fosmid clones has been determined as described in the related publication.

DOI https://doi.org/10.1594/PANGAEA.937551
Related Identifier https://doi.org/10.3389/fbioe.2020.00567
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.937551
Creator Adam, Nicole; Schlicht, Stefanie; Han, Yuchen; Bechelany, Mikhael; Bachmann, Julien; Perner, Mirjam
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2021
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 95 data points
Discipline Earth System Research
Spatial Coverage (-13.509W, -9.544S, -12.372E, -4.804N)
Temporal Coverage Begin 2009-04-17T18:08:00Z
Temporal Coverage End 2009-04-30T11:50:00Z