Magnetic contrast reflectometry to resolve transmembrane potential effects on the binding of MinD-MTS to a floating bilayer

DOI

The division of bacteria is controlled by the binding of proteins to the inner of the two lipid bilayer membranes that separates the inside of the cell from the outside world. This membrane behaves like a capacitor and its properties are thought to vary with the size of the electrical potential that exists across it. We can construct a flat model for this membrane above a gold-coated silicon block and use the reflection of neutrons from this thin film to probe the details of the structure of the membrane and how this changes with an applied electrical potential and when the anchoring peptide of the cell division protein inserts into the membrane. From this information we hope to understand if the insertion of this protein is controlled by the electrical potential, which can be related to metabolic state of actual bacteria. In the long term this may help to control bacterial infection.

Identifier
DOI https://doi.org/10.5286/ISIS.E.58448079
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/58448079
Provenance
Creator Dr Timothy Charlton; Dr Simon Titmuss; Dr Iva Manasi; Dr Rob Barker; Miss Laura McKinley
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2015-03-29T07:00:00Z
Temporal Coverage End 2015-04-02T07:00:00Z