Enterococcus faecalis is a Gram-positive opportunistic pathogen that inhabits the gastrointestinal tract. Because of the high frequency of antibiotic resistance among Enterococcus clinical isolates, interest in using phage to treat enterococcal infections and to decolonize high-risk patients for antibiotic-resistant Enterococcus is rising. Bacteria can evolve phage resistance, but there is little published information on these mechanisms in E. faecalis. In this report, we identified genetic determinants of resistance to phiNPV1 and their effects on E. faecalis susceptibilities to daptomycin and sodium chloride-induced osmotic stress.