Time series of stable isotopes (δ2H and δ18O) were analyzed in water samples collected at the Zingster Bodden chain in biweekly to monthly intervals between March 2020 and March 2021. Herefore, a Limnos sampler was used to obtain water from 0.5 to 1 m below surface. In the laboratory, a WTW 1970i conductivity meter and TetraCon 325 measuring cell were used to analyse electrical conductivity. Sub-samples were transferred into measurement vials before isotope analysis was conducted at IGB Berlin, using a Picarro L2130-i cavity ring-down spectrometer. Water chemical parameters were measured with regularly calibrated WTW multiparameter devices.The data give information about the seasonal isotope amplitude at the sampled locations and about spatial variability along the transects.