Lipid Exchange in Polymer Stabilized Lipid Nanodiscs with Varying Curvature

DOI

Styrene-Maleic Acid Lipid Particles (SMALPs) are stable nano-aggregates of polymer and lipid which self-assemble into monodisperse discs in solution. They have been shown to solubilize membrane proteins which gives them potential to assist in membrane protein structure determination. Our recent studies of these discs suggest the disc curvature can be modified by the lipid mixtures used. Since curvature affects membrane protein structures and function we therefore wish to explore the properties of our nanodiscs by studying lipid exchange between discs of selected curvature, induced either by lipid charge or by altering tail volumes. Lipid exchange will be probed by following changes in scattered intensity with time when solutions containing h-lipid discs and d-lipid discs are mixed. Following exchange rates at different temperatures will allow the thermodynamics of lipid exchange in this polymer stabilized system to be compared with studies by others on protein stabilized nanodiscs and liposomes. This work will form the basis of future structure/function studies on proteins in lipid nanodiscs with controlled curvature.

Identifier
DOI https://doi.org/10.5291/ILL-DATA.9-13-468
Metadata Access https://data.ill.fr/openaire/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=10.5291/ILL-DATA.9-13-468
Provenance
Creator Neylon, Cameron; Hazell, Gavin; Idini, Ilaria; Grillo, Isabelle; Edler, Karen; Schweins, Ralf
Publisher Institut Laue-Langevin
Publication Year 2014
Rights OpenAccess; info:eu-repo/semantics/openAccess
OpenAccess true
Representation
Resource Type Dataset
Size 3 GB
Version 1
Discipline Particles, Nuclei and Fields