This dataset contains the infrared spectra of BJ human foreskin fibroblasts (healthy) and B16-F10 mouse skin melanoma (tumour) cell lines subjected to standard neon radiotherapy and neon minibeam radiation therapy (NeMBRT), measured via synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-FTIRM)

METHODOLOGICAL INFORMATION

1. Description of methods used for collection-generation of data: BJ human foreskin fibroblasts (healthy) and B16-F10 mouse skin melanoma (tumour) cell lines, previously subjected to standard neon radiotherapy or neon minibeam radiation therapy (Heavy Ion Medical Accelerator in Chiba, HIMAC, Japan), were fixated on infrared transparent calcium fluoride coverglasses, suitable for infrared microspectroscopy measurements. Fixation of samples occurred at both 0 hours and 24 hours post-treatment. The protocol is detailed in [González-Vegas, et al. (2025). Analyst, 150, 342–352]. The SR-FTIRM measurements were conducted at the MIRAS beamline of ALBA Synchrotron (Cerdanyola del Vallès, Spain). Over 125 cells were randomly selected for each sample and irradiation configuration (control, broad beam, minibeam peak, minibeam valley). Single-cell infrared measurements were collected in the 3800-1000 cm-1 mid-infrared spectral range, using a spectral resolution of 4 cm-1 and 256 co-added scans per spectrum

2. Methods for processing the data: Spectral data was cut into two distinct spectral ranges: the Higher Wavenumber (HW, 3000–2800 cm-1) or Amides and Fingerprint (AFP, 1800–1000 cm-1). In both spectral regions, data was processed using a Savitzky-Golay filter (2nd derivative order; 9 points window in the HW region, 19–25 points window in the AFP region) followed by unit vector normalization. Data processing was performed with the Quasar software (version 1.9)

3. Instrument- or software- specific information needed to interpret the data: Any software able to read csv files

4. Instruments, calibration and standards information: Hyperion 3000 microscope, Vertex 70 Spectrometer (Bruker Optics GmbH)

5. Environmental or experimental conditions: SR-FTIRM measurements were carried out on dried cells at room temperature. Background spectra were collected every 5 samples to compensate for varying ambient conditions inside the MIRAS beamline

6. Quality-assurance procedures performed on the data: No