PLN (phosphatidylnucleosides) are phospholipid-based anionic nucleolipids, where a nucleoside is enzymatically attached to thepolar head of a lecithin substituting a choline headgroup. In the past years several studies have highlighted that the microstructure oftheir self-assemblies depends markedly on the kind of nucleic base due to inter polar-head attractive interactions and, moreover,that PLN self assemblies provide negatively charged interfaces decorated with nucleic motifs that can complex complementarynucleic acid strands using, as the driving force, molecular recognition instead of electrostatic interactions. Neutron reflectometrytechnique can allow a specific investigation on the interaction between nucleolipids' membranes and different (i.e. characterized bydifferent length and nucleobase sequence) single strand nucleic acids, allowing hence to better comprehend the molecularrecognition interaction between PLN bioinspired amphiphiles and nucleic acids.