Probing the dynamics of Thermococcus barophilus under high temperature and high pressure

DOI

The majority of the biosphere is a high pressure environment. Around 70% of the marine biosphere lies at depths below 1000 m, i.e. at pressures of 100 bars or higher. To survive in these environments, deep-biosphere organisms have adapted to life at high pressure. In vitro studies showed that the activity of certain proteins originating from deep-sea organisms is less affected by high pressure than that of enzymes from surface organisms. However, the genetic and structural bases for this increased pressure resistance are still unknown. Therefore we want to investigate high pressure effects on cell dynamics using Thermococcales as models. Former experiments on two of the species showed the influence pressure has on these microbes, but to date only measurements at 300 K could be done, as the high pressure cell did not withstand high temperature. As these organisms life close to hot vents we developed a new cell and want to repeate now a QENS measurement at native temperature conditions (360 K) to be compared to the former results. For that we would imperatively need IN5 to stay in the same instrumental conditions.

Identifier
DOI https://doi.org/10.5291/ILL-DATA.8-04-783
Metadata Access https://data.ill.fr/openaire/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=10.5291/ILL-DATA.8-04-783
Provenance
Creator Cario, Anais; Ollivier, Jacques; Peters, Judith; Golub, Maksym; Castell, Marta Salvador; Oger, Philippe
Publisher Institut Laue-Langevin
Publication Year 2017
Rights OpenAccess; info:eu-repo/semantics/openAccess
OpenAccess true
Representation
Resource Type Dataset
Size 12 GB
Version 1
Discipline Particles, Nuclei and Fields