10-days old mycelium of B. cinerea wild type (WT) and dcl1dcl2 mutant grown on solid malt extract agar plates were used for RNA isolation. Total RNAs was extracted using the Trizol® method. Small RNA fraction of 18-40 nucleotides was isolated by 14 % 8 M Urea PAGE. Small RNA library were cloned following the miRCat small RNA cloning system (IDT, Coralville, IA).