Raw data from a series of experiments aimed to evaluate various agricultural byproducts hydrolysates, including wheat feed, rapeseed meal, coconut waste, and palm waste, to assess their suitability as nitrogen sources for SL production, with the subsequently scale-up of the process.

METHODOLOGICAL INFORMATION

Experimental work involved submerged fermentation (SmF) using Starmerella bombicola yeast. Four byproducts were hydrolyzed and subsequently employed as nitrogen sources. The hydrolysates that presented the highest SL production were selected to evaluate several total nitrogen concentrations, and the optimal combination was then scaled-up in a 2-L bioreactor.

1. Description of methods used for collection-generation of data: Lab scale experiments were carried out using 4-bottom baffled 250 mL Erlenmeyer flasks with a total working volume of 50 mL media. Then, a time course fementation was performed at 2-L Batch bioreactor.

2. Methods for processing the data:

3. Cell dry weight results were analized by gravimetrical quantification.
4. SL concentration and remaining oil were analytically determined after solvents extraction using ethyl acetate and n-hexane.

5. Diacetylated lactonic C18:1 and glucose concentration was quantified by HPLC-UV.

6. Instrument- or software- specific information needed to interpret the data:

7. Excel or similar soft.

8. JMP® 15 statistical software (JMP Statistical Discovery LLC, USA).

9. Instruments, calibration and standards information:

10. Analytical balance Sartorius Entris II
11. Orbital shaker (Infors HT, UK)
12. Centrifuge (Sigma 6–16S, SciQuip, UK).
13. HPLC UltiMateTM 3000
14. Macherey-Nagel™ Nucleosil™ 100 mm × 3 μm x 4.7 mm C18 EC column
15. Aminex HPX-87 P column (Biorad, USA) coupled to a refractive index detector (RI)
16. Multi-N/C 2100S analyzer (Analytik Jena, INYCOM, Instrumentación y Componentes, S.A, Spain)

17. 2-L bioreactor (BioStat® B, Sartorius, Germany)

18. Environmental or experimental conditions:

19. Starmerella bombicola ATCC 22214 was cryopreserved at -80°C.
20. Microorganism growing conditions were set up at 30 ºC, 200 rpm at 24 h.
21. Hydrolisis conditions were 55°C, 24 h and 200 rpm.
22. Fermentations in 4-bottom baffled 250 mL Erlenmeyer flasks were conducted at 30 ºC, 168 h, and 200 rpm.

23. 2-L bioreactor operational parameters were established at: temperature 30 ºC, airflow rate 2 mL/min, dissolved oxygen level was consistently maintained above 30% and regulated by adjusting the agitation speed within the range of 200 to 800 rpm.

24. Quality-assurance procedures performed on the data: All experiments were carried out in duplicate to estimate pure error.